Buffer A

Need 30mL/run + 200mL

For 2L:

• 3.74g monobasic KH₂PO₄
• 1.44mL H₃PO₄
• HPLC water to 2L

Buffer B

Need 15mL/run + 200mL

For 1L:

• 50mL THF
• 950mL HPLC MeOH